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KMID : 0363620090300040013
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Journal of Korean Oriental Medicine
2009 Volume.30 No. 4 p.13 ~ p.27
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The Effect of Trans-cinnamaldehyde on the Gene Expression of Lipopolysaccharide-stimulated BV-2 Cells Using Microarray Analysis
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Sun Young-Jae
Jung Mi-Young
Hwang Se-Hee
Lee Je-Hyun
Cho Jung-Hee
Lim Sabina
Chio Yeong-Gon
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Abstract
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Objectives: Trans-cinnamaldehyde (TCA) is the main component of Cinnamomi Ramulus and it has been reported that TCA inhibits inflammatory responses in various cell types. Inflammation-mediated neurological disorders induce the activation of macrophages such as microglia in brain, and these activated macrophages release various inflammation-related molecules, which can be neurotoxic if overproduced. In this study, we evaluated gene expression profiles using gene chip microarrays in lipopolysaccharide (LPS)-stimulated BV-2 cells to investigate the antiinflammatory effect of TCA on inflammatory responses in brain microglia.
Methods: A negative control group was cultured in normal medium and a positive control group was stimulated with in the absence of TCA. TCA group was pretreated with before LPS stimulation. The oligonucleotide microarray analysis was performed to obtain the expression profiles of 28,853 genes using gene chip mouse gene 1.0 ST array in this study.
Results: In positive control group, 1522 probe sets were up-regulated in the condition of the cutoff value of 1.5-fold change and 341 genes with Unigene ID were retrieved. In TCA group, 590 probe sets were down-regulated from among 1522 probe sets and 33 genes with Unigene ID were retrieved, which included 6 inflammation-related genes. We found out that Id3 gene is associated with transforming growth factor- (TGF-) signaling pathway and Klra8 gene is related to natural killer cell-mediated cytotoxicity pathway.
Conclusions: The results mean that TCA inhibits inflammatory responses through down-regulating the expressions of inflammation-related genes in LPS-stimulated BV-2 cells.
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KEYWORD
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trans-cinnamaldehyde, lipopolysaccharide, microglia, BV-2, microarray
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